https://vibe-genomics.replit.app/assets/wetlab-BPPx-tpM.jpg
Unfortunately I did already sell my soul to 23andme a long time ago, but this did help validate that my workflow worked as a ground truth! I took one for the team here privacy wise haha
I used an Oxford Nanopore MinIon sequencer, a Zymo miniprep DNA extraction kit, the ONT Rapid Sequencing kit, and 3 ONT flow cells to hit about 16x coverage
I checked it against my 600k 23andme SNPs and it held up!
I think there is a lot of waste here and a lot of process refinement that would make this more accessible, but hopefully this is a good starting point if anyone wants to do this themselves!
Claude helped break down what the different phases I needed to follow were, searched out the most straightforward workflows/kits for DNA collection, extraction, library prep, and sequencing, pulled all the protocols into one place and adapted the workflows to the equipment I had (and it made some apparently good guesses to help me skip quantification), then did 100% of the secondary analysis!
Crazy how you can just “vibe genomics” this stuff these days
